Workshops

Pre-meeting Workshops

2 pre-meeting workshops will take place on Saturday, July 30th in the Elm Ballroom at the SMC Campus Center.

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Pre-Meeting Workshop 1: MSiReader Pro: An Intuitive and Innovative Vendor Neutral Software for Mass Spectrometry Imaging

Saturday, July 30th - 1:00pm-3:00pm

Current software solutions for analyzing mass spectrometry imaging (MSI) data are either cost-prohibitive and vendor-specific, i.e., optimized for proprietary data formats, and/or lack diverse functionality, ease-of-use, documentation, and technical support. This workshop will cover basic functions within MSiReader Pro as well as several advanced functions; MSiReader Pro is vendor-neutral and a user-centric solution providing a diverse array of advanced tools. MSiReader Pro reader enables researchers to turn raw MSI data into biological insights, helping accelerate biomedical innovations that positively affect human health. Sufficient time will be dedicated to discussion so we can answer specific questions and discuss future algorithms or changes to better support the MSI community.

Pre-Meeting Workshop 2: Strategic Design of MALDI Imaging Sample Preparation

Saturday, July 30th - 3:00pm-4:00pm

This workshop is designed for scientists who want to learn and refine techniques for strategic design of MALDI imaging sample preparation, with an emphasis on high spatial resolution experiments. Advantages and limitations to various methods of sample preparation will be described and compared, including starting material, matrix choice, matrix application, and enzyme workflows. Factorial experimental design as well as non-statistical approaches will be presented. Finally, real-world applications to design of tissue and non-tissue imaging projects will be reviewed, along with discussions of rigorous sample preparation reporting strategies.

Meeting Workshops

All meeting workshops will take place in the Elm Ballroom at the SMC Campus Center.

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Workshop 1: Multimodal IMS: Concepts and Practicalities of Integrating IMS, Microscopy and Other Data

Sunday, July 31st - 3:00pm-5:00pm

Workshop Organizer:

This workshop will discuss fundamental concepts and practicalities of how researchers can best use microscopy (and other modalities) to guide their IMS data analysis or data acquisition. We will discuss and demonstrate topics such as image file formats, image registration, microscopy-guided image analysis, and more. In addition, we will discuss some advanced methods employed at the MassSpectrometry Research Center to achieve high accuracy image registration and powerful image analysis.

Workshop 2: Integration of metabolomics with transcriptomic and proteomic datasets: The whole is greater than the sum of its parts

Sunday, July 31st - 3:00pm-5:00pm

Workshop Organizer:

With the advent of high-throughput technologies, biological experiments have shifted from generating small datasets that focus on single genes or proteins to generating large data sets that document expression profiles on a whole-genome wide basis. Similarly, metabolomicsvtechnologies allow quantification of lipids, glycans and other molecules on a similar scale,leading to the generation of large metabolomics expression profiles. Each of the transcriptomic,proteomic and metabolomics assays offers a different view on the investigated biology that can support findings by the other assays and give additional complementary insights. Integration strategies that map the findings across different assays can help to maximize the insight obtained by the different assays. Integration between metabolomic and transcriptomic or proteomic technologies can be achieved on multiple levels. On the lowest level a researcher can investigate if the transcript omic or proteomic datasets identify enzymes that either directly catalyze reactions of the identified metabolites or of closely related precursors and derivatives.On the second level a researcher can analyze if a set of metabolites maps to a pathway that was identified by the other omic datasets as well. On the highest level a researcher can finally investigate if pathways that were identified for the metabolites are functionally related to pathways identified based on other omic assays. Here, we will discuss the integration of metabolomics with single cell and bulk RNA seq and proteomic datasets. We will investigate theintegration strategies on each of the three different levels. We will also discuss an example where the integration of metabolomics with other omic datasets and prior biological knowledge allows hypothesis generation about function and relevance of a major metabolomics finding.

Workshop 3: An Introduction to SIMS, DESI, and LASEI for Mass Spectrometry Imaging (Part A)

Tuesday, August 2nd - 1:00pm-3:00pm

Workshop Organizer:

Dr. Gregory L. Fisher is the Principal Scientist at Physical Electronics, and the Product& Marketing Manager for time-of-flight secondary ion mass spectrometry (TOF-SIMS).Greg earned B.S. degrees in Chemistry and Physics at the University of Wisconsin at LaCrosse, and a Ph.D. at the Pennsylvania State University. Greg was at Los AlamosNational Laboratory for seven years engaged in the high explosive reaction chemistry via ultrafast laser-excited spectroscopy (HERCULES) project and was part of the nuclear materials science group contributing to LANL’s nuclear materials mission. While atLANL, Greg was at the forefront of cluster ion beam development and established the use of a C60 ion beam in high sensitivity measurements of materials chemistry. Since joining PHI in 2006, an underlying theme of Greg’s work has been the development ofTOF-SIMS as a practical tool for molecular and high m/z ion identification in materials, pharmaceutical, and bio-medical research with applications including restenosis, oncology and neuroplasticity. Greg’s work has led to a number of innovations and patents. A hallmark has been the development of a new mass spectrometer technology forParallel Imaging MS/MS (TOF-SIMS tandem MS imaging) which enables the compositional and structural elucidation of molecules in a lossless fashion and with visualization at < 100 nm resolution.

Greg’s lecture at the IMSS Workshop 2022 will touch on the fundamental physics, engineering design considerations, and analytical applications of TOF-SIMS with an overview of TOF-SIMS fit with respect to other methods of mass spectrometry analysis and surface characterization. Succinct and practical introductions will be provided on topics including: ion/surface interactions, energetics and ion formation, time-of-flight principles, mass spectrometer designs, ion column designs and analytical ion beam characteristics, and applications involving 1D, 2D and 3D characterization.

See more about Dr. Gregory L. Fisher via LinkedIn.

DESI and Ambient Ionization (Part B)

Desorption electrospray ionization (DESI) is one of the widely used imaging mass spectrometry (MS) techniques. Ambient ionization sources, such as DESI, are capable of direct analysis of samples with minimal sample preparation and, thus inherently suited to serve as an imaging MS ion source. This workshop will provide an overview of DESI and other ambient ion sources, such as laser ablation electrospray ionization (LAESI), used for imaging MS. The workshop will contain an in-depth discussion on DESI ion source; a step-by-step tutorial on DESI imaging software for acquiring and processing data; several examples of DESI for imaging MS applications; and a comparison with MALDI. For further questions, contact Bindesh Shrestha at bindesh_shrestha@waters.com.

Workshop 4: Government Funding Workshop

Tuesday, August 2nd - 1:00pm-3:00pm

This Government Funding Workshop will serve two purposes: (1) provide our attendees with information about how to secure funding from the funding agencies represented and (2) provide a networking space to discuss applications and new research opportunities for imaging mass spectrometry. This workshop will include a panelist Q&A and then a structured networking event.

Workshop Organizer: Colleen Crouch (acrouch5@tennessee.edu)

Program Directors/Managers:

Kelly Crotty, PhD, Program Director, National Cancer Institute, NIH

Paul Sammak, PhD, Program Manager, Biological and Environmental Research, Office of Science, U.S. Department of Energy

Eric Brunskill, Program Director, NIDDK, NIH (pre-recorded)
Joan Greve, PhD, Program Director, SBIR, National Cancer Institute, NIH (Virtual)

Scientists:

C. Linn Cadieux, PhD, Research Chemist, U.S. Army Medical Research Institute of Chemical Defense

E. Ellen Jones, PhD, Research Biologist, National Center for Toxicological Research/FDA, Division of Systems Biology; Omics, Modeling, Imaging and Chemistry (OMIC) Branch