(210001DJ) A Laboratory Research Assistant Position is immediately available in the Scott Lab at the University of Maryland, Baltimore School of Dentistry (SOD) in the Department of Microbial Pathogenesis. The lab studies the role(s) of lipids (engineered or native) in the early innate immune response with an aim to alleviate conditions linked to uncontrolled inflammation. The funded project joins elements of neuroimmunology and pain biology and will test new ways to control pain through immunomodulation. This Research Assistant will participate in exciting and diverse aspects of the project: 1) biochemical extraction, purification, and structural characterization of glycolipids; 2) assisting with rodent behavioral modeling and colony maintenance; 3) exploration of molecular mechanisms of pain using basic techniques such as ELISA, immunohistochemistry, western blotting, and qRT-PCR. The ideal candidate will have experience in microbiology, immunology, neurobiology, biochemistry, or related areas with some documented experience in the techniques listed here. While previous experience working with rodents in a research setting is ideal, willingness to be trained on and work with these models is a requirement. This position will be a critical member of a collaborative, dynamic team and must have good oral and written communication skills and will be expected to be self-motivated to learn and perform independently.

This is a FULL-TIME, CONTRACTUAL position,

UMB was ranked one of America's Best Large Employers by Forbes Magazine (Feb 2021).

UMB offers a comprehensive and competitive benefits package to eligible employees. This is a contractual position that offers a generous leave package of 10 vacation days, 3 personal days, 8 holidays, and 5 sick days; comprehensive health insurance and supplemental retirement options; professional learning and development opportunities; and limited tuition remission for employees enrolled at UMB.

UMB requires all faculty, staff, and students to be vaccinated against COVID-19. Exemptions for medical or religious reasons will be processed through Human Resources.  

Primary Duties:

• Perform routine laboratory maintenance and research activities such as: general housekeeping, wipe tests, ELISA assays, preparing solutions, media, stains, and buffers; cultures and specimens; autoradiograms and photomicrographs, executing biochemical procedures such as: electrophoresis, blotting, chromatography, and DNA extractions, etc.
• May perform advanced laboratory research activities such as: animal surgery, microscopy techniques, imaging techniques, recombinant DNA techniques, protein purification, and hybridizations, etc. May participate in the design of research studies.
• Compile and may analyze research data using various relevant computer software applications.
• Assist with literature research, editing and preparing manuscripts, scientific illustrations and computer graphics for publication.
• Perform administrative duties such as: training and guiding students and/or other laboratory technicians; assisting in developing policies, procedures and/or methods for laboratory experimentation; and ordering supplies.
• Perform other duties as assigned.

 

Qualifications

  

Education: Bachelor's degree in biology, chemistry or field of study related to the research of the laboratory.

Other: May require training related to occupational safety and health, environmental compliance, shipping of hazardous materials and/or ionizing radiation.

May consider a combination of directly related experience and education.

Knowledge, Skills, and Abilities

• Knowledge of position requirements. Knowledge of all applicable requirements, regulations, and laws. Skill in effective use of applicable technology/systems. Ability to effectively communicate both verbal and written thoughts, ideas, and facts. Ability to work cooperatively with others and independently. Ability to demonstrate, understand, apply, and adhere to the UMB Core Values of Accountability, Civility, Collaboration, Diversity, Excellence, Leadership, and Knowledge.

Preferred Knowledge, Skills, and Abilities:

1. Immunohistochemistry

2. ELISA

3. qRT-PCR

4. Biochemical extraction

5. Rodent handling

Hiring Range: Commensurate with education and experience

UMB is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to sex, gender identity, sexual orientation, race, color, religion, national origin, disability, protected Veteran status, age, or any other characteristic protected by law or policy. For assistance related to employment, please contact the Staffing department at HRJobs@umaryland.edu.

If you anticipate needing a reasonable accommodation for a disability under the Americans With Disabilities Act (ADA), during any part of the employment process, please submit a UMB Job Applicant Accommodation Request. You may also contact HRDiversity@umaryland.edu. Please note that only inquiries concerning an ADA request for reasonable accommodation will be responded to from this email address.

 

Job

: Reg or CII Exempt Staff - E3303C

Employee Class Exempt Contingent II

Full Time/Part Time: Full-Time

Shift

: Day Job

Financial Disclosure: No

Organization

: School of Dentistry - Microbial Pathogenesis

Job Posting

: Oct 6, 2021

Unposting Date

: Oct 27, 2021, 9:59:00 PM

Postdoctoral Research Fellow - Vanderbilt University: The laboratory of Jeff Spraggins in the Mass Spectrometry Research Center and Department of Cell & Developmental Biology at Vanderbilt University is looking for outstanding postdoctoral candidates that will join a team of graduate and post-graduate researchers to develop multimodal 2-D and 3-D imaging mass spectrometry. Multiple projects are available, including developing MALDI imaging instrumentation, spatially driven multi-omics, tissue processing and preparation methods, and computational data integration and mining tools. These projects involve constructing molecular atlases of human tissue and understanding the molecular drivers of host-pathogen interactions.

Post-doctoral candidates joining the Mass Spectrometry Research Center will have access to state-of-the-art instrumentation, including 9T and 15T Bruker Fourier transform ion cyclotron mass spectrometers with MALDI and ESI sources, two Bruker timsTOF ion mobility/Q-TOF mass spectrometers with MALDI and ESI capabilities, a Bruker Rapiflex MALDI tissuetyper, and a Thermo Orbitrap Elite mass spectrometer with a Spectroglyph transmission geometry source capable of 1 micron imaging. We also have high-performance LC-MS/MS instrumentation including a Thermo Orbitrap Fusion Tribrid mass spectrometer, a Bruker Impact II Q-TOF with a nanoElute LC system, and an Agilent AssayMAP Bravo liquid handler. Multimodal and microscopy-based imaging instrumentation include a GeoMX Digitial Spatial Profiler, a Zeiss Axio Observer Z1 inverted microscope integrated with an Akoya CODEX system, and a Zeiss Axio Scan Z1 brightfield and fluorescence slide scanner. The Mass Spectrometry Research Center also hosts a collection of commercial and state-of-the-art custom software for analysis of imaging, multiomics, and microscopy data.

Post-doctoral candidates will benefit from faculty with a diverse range of expertise including chemistry, biochemistry, engineering, computation, and biology. The Mass Spectrometry Research Center at Vanderbilt University is a leading institution for the development of imaging mass spectrometry and multimodal technologies providing a systems biology view of biological systems at cellular resolution.

Requirements: MALDI imaging, LC-MS, immunohistochemistry, and/or transcriptomics skills are required. Skills that are a plus, but not required, include instrumentation development, programming/scripting, bioinformatics, image processing and visualization, knowledge in pathology and infectious disease, ability to work simultaneously on multiple projects (including internal and external collaborations), and strong willingness to learn additional research skills if needed.

Location: Nashville, Tennessee, United States

Contact: If interested please email Jeff Spraggins at jeff.spraggins@vanderbilt.edu

Drug Metabolism and Distribution group within the DMPK department of Abbvie utilizes MS imaging to help drive molecules to the market. The successful candidate will pursue several research initiatives that will enhance the utility of MSI for tissue distribution and understanding of safety.  One area is to increase the sensitivity of imaging by utilizing novel ion source and targeted imaging methodologies to allow projects to be investigated when analytes fall below the current limit of detection. It is also important to Identify endogenous markers in various tissues (eye, liver, kidney, heart, etc.) to provide a high confidence of assignment in tissue distribution analysis. The generation of ion atlas will broadly improve spatial specificity, accelerate biomarker discovery for drug development, and significantly increase the ability to fully investigate the molecular drivers of biological processes. In addition, large molecule imaging methods and improved protocols need to be developed for MS imaging to be more robust and routine. This would permit the distribution of ADCs, antibody, and anticancer agents to be more fully investigated by MS imaging spectrometry.

please apply the position through Abbvie's site:

https://careers.abbvie.com/abbvie/jobs/2100676?lang=en-us&previousLocale=en-US

Investigator, MALDI MS Bioimaging (myworkdayjobs.com)

We have an exciting new position available within the MALDI MS Bioimaging group at GSK, based from our world-class R&D site at Collegeville, PA.  The role will focus on development of non-targeted imaging mass spectrometry-based workflows to deliver functional pharmacology mechanistic endpoints to projects.  As part of the MALDI MS Bioimaging team, you’ll be responsible for the application and advancement of imaging mass spectrometry (IMS) to better understand the mechanisms of drug tissue disposition, pharmacokinetics, pharmacology, and toxicology in the development of safe and efficacious drugs.  Utilizing leading edge MS instrumentation, custom imaging software tools, and pioneering IMS tissue quantification, our innovative group works collaboratively to help drive critical decisions in all therapeutic areas. 

Bioimaging at GSK

The Bioimaging department brings together key areas of in vivo and ex vivo imaging combined with histology to understand both disease state and intervention, combining data on drug distribution with the effect that distribution has on intact biological systems.  At GSK we believe that the fewer barriers there are in delivering the science, then the better our delivery of new medicines to patients. As part of the wider In Vitro/In Vivo Translation platform we encourage enterprise collaboration as well as external partnership with the wider scientific community to achieve our full potential.

Key Responsibilities:

• Develop non-targeted IMS workflows to deliver functional pharmacology mechanistic endpoints for projects and enhance understanding of biological systems

• Develop molecular identification strategies using tissue extracts and tissue surface analysis methods (e.g. LESA) to achieve accurate molecular assignment from MALDI imaging data

• Work in partnership with the Imaging Analytics teams and other stakeholders to build data repositories and custom pipelines to achieve streamlined identification of endogenous biological molecules and cellular pathways, and facilitate broader data reuse

• Work in partnership with project teams to understand project issues, provide input and report data to matrix teams, including close networking with all imaging groups across GSK.

• Maintain timely communication to named key study personnel, other key stakeholders and management, of study related events and/or deviations.

• Share responsibility for lab management, equipment maintenance, safety rep and similar roles.

Why you?

Basic Qualifications:

We are looking for professionals with these required skills to achieve our goals:

• PhD in Chemistry, Biology, Physical Sciences, or related discipline.

• Experience in mass spectrometry-based metabolomics and/or lipidomics data acquisition and analysis

• Familiarity and experience with common identification strategies for endogenous biological molecules

• Experience with troubleshooting and maintaining complex instrumentation

Preferred Qualifications:

If you have the following characteristics, it would be a plus:

• Expertise in imaging mass spectrometry

• Experience with Thermo or Bruker mass spectrometers

• Evidence of effective, collaborative interpersonal, organizational and communication skills, both oral and written, for working in a multi-disciplinary scientific team environment.

Why GSK?

Our values and expectations are at the heart of everything we do and form an important part of our culture. These include Patient focus, Transparency, Respect, Integrity along with Courage, Accountability, Development, and Teamwork. As GSK focuses on our values and expectations and a culture of innovation, performance, and trust, the successful candidate will demonstrate the following capabilities:

• Operating at pace and agile decision-making – using evidence and applying judgement to balance pace, rigour and risk.

• Committed to delivering high quality results, overcoming challenges, focusing on what matters, execution.

• Continuously looking for opportunities to learn, build skills and share learning.

• Sustaining energy and well-being

• Building strong relationships and collaboration, honest and open conversations.

• Budgeting and cost-consciousness

*LI-GSK

If you require an accommodation or other assistance to apply for a job at GSK, please contact the GSK Service Centre at 1-877-694-7547 (US Toll Free) or +1 801 567 5155 (outside US).

GSK is an Equal Opportunity Employer and, in the US, we adhere to Affirmative Action principles. This ensures that all qualified applicants will receive equal consideration for employment without regard to race, color, national origin, religion, sex, pregnancy, marital status, sexual orientation, gender identity/expression, age, disability, genetic information, military service, covered/protected veteran status or any other federal, state or local protected class.

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GSK does not accept referrals from employment businesses and/or employment agencies in respect of the vacancies posted on this site. All employment businesses/agencies are required to contact GSK's commercial and general procurement/human resources department to obtain prior written authorization before referring any candidates to GSK. The obtaining of prior written authorization is a condition precedent to any agreement (verbal or written) between the employment business/ agency and GSK. In the absence of such written authorization being obtained any actions undertaken by the employment business/agency shall be deemed to have been performed without the consent or contractual agreement of GSK. GSK shall therefore not be liable for any fees arising from such actions or any fees arising from any referrals by employment businesses/agencies in respect of the vacancies posted on this site.

Please note that if you are a US Licensed Healthcare Professional or Healthcare Professional as defined by the laws of the state issuing your license, GSK may be required to capture and report expenses GSK incurs, on your behalf, in the event you are afforded an interview for employment. This capture of applicable transfers of value is necessary to ensure GSK’s compliance to all federal and state US Transparency requirements. For more information, please visit GSK’s Transparency Reporting For the Record site.